MDL No.: MFCD03458197
Storage: -70C
UNSPSC Code: 12352200
General description: Manufactured for Sigma by Boston Biochem., Inc.
Application: Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.
Biochem/physiol Actions: Protein fraction of a cell extract that binds to anion-exchange resin.
Biochem/physiol Actions: Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.
Physical form: Solution in 25 mM HEPES, pH 7.6.
RIDADR: NONH for all modes of transport
WGK Germany: 2