Synonym(s): Actinase E; Pronase E
CAS No.: 9036-06-0
Application: This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.
Physical properties: Completely inactivated by heating above 80 °C for 15-20 minutes.
Preparation Note: Collected from culture broth of S. griseus and purified by successive column procedures.
Other Notes: An unusually non-specific protease.
Storage: −20°C
EC No.: 232-909-5
Quality: Contains approximately 25% calcium acetate.
Specificity: A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.
Unit Definition: One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).