Synonyms: Cytosolic Protamine Kinase
MDL No.: MFCD04041070
Purity: >90% (SDS-PAGE)
Activity: activity: >=15,000 units/mg protein
Storage: -70C
UNSPSC Code: 12352204
Application: Cytosolic Protamine Kinase (cPK) is isolated from bovine kidney and has a molecular mass of approximately 45 kDa. It phosphorylates the eukaryotic Initiation Factor 4E (eIF4E), which initiates translation by mRNA. It is used to study protein synthesis and various cellular processes.
Biochem/physiol Actions: Cytosolic protamine kinase is involved in the regulation of protein synthesis and is indirectly associated with numerous cellular processes. Cytosolic protamine kinase is a distinct insulin-stimulated kinase involved in the phosphorylation of eukaryotic Initiation Factor 4E (eIF4E) which is key to initiating translation by mRNA. This protein appears to be inactivated by protein phosphatase 2A family members and may also be inhibited by microcystin, okadeic acid, and ATP. The phosphorylation process is reversible and MBPK1 and MBPK2 (Myelin Basic Protein Kinase 1 and 2) may reactivate cytosolic protamine kinase .
Physical form: Solution in 50 mul of 50 mM Tris-HCl, pH 7.0, containing 14 mM beta-mercaptoethanol, 1mM benzamidine, 0.1 mM PMSF, 1mM EDTA, 50% glycerol
Unit Definition: One unit will incorporate 1 nmol of phosphate using protamine sulfate as a substrate per minute at pH 7.0 at 30 C.