PNGase F from Elizabethkingia meningoseptica, recombinant, expressed in E. coli, set of 100 units nanomolar unit

Synonyms: N-Glycanase(R); N-Glycosidase F; PNGase F from Chryseobacterium meningosepticum; PNGase F from Flavobacterium meningosepticum; Peptide N-glycosidase

CAS Number: 83534-39-8

MDL No.: MFCD00131222

Activity: activity: >=10 units/mg protein

Storage: 2-8C

Enzyme Commission (EC) Number: 3.5.1.52  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Application: Used to deglycosylate protein.

Application: PNGase F is a glycosylasparaginase used to deglycosylate proteins. It is widely used in structure-function studies of glycoproteins.

Biochem/physiol Actions: Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Biochem/physiol Actions: PNGase F cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact. PNGase F will not remove oligosaccharides containing α(1-3)-linked core fucose, commonly found in plant glycoproteins. A tripeptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F.

Unit Definition: One unit will catalyze the release of N-linked oligosaccharides from 1 micromole of denatured ribonuclease B in one minute at 37C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.