Synonyms: N-Glycosidase F; PNGase F from Chryseobacterium meningosepticum; PNGase F from Flavobacterium meningosepticum; Peptide N-glycosidase
CAS Number: 83534-39-8
MDL No.: MFCD00131222
Activity: activity: >=1,000 U/mg
Storage: -20C
Enzyme Commission (EC) Number: 3.5.1.52 ( BRENDA | IUBMB )
UNSPSC Code: 12352200
Application: Used to deglycosylate protein.
Application: Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.
Biochem/physiol Actions: Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.
Unit Definition: One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.
RIDADR: NONH for all modes of transport
WGK Germany: 3