PNGase F from Elizabethkingia meningoseptica, BioReagent, >=95% (SDS-PAGE), for proteomics

Synonyms: N-Glycosidase F; PNGase F from Chryseobacterium meningosepticum; PNGase F from Flavobacterium meningosepticum; Peptide N-glycosidase

CAS Number: 83534-39-8

MDL No.: MFCD00131222

Purity: >=95% (SDS-PAGE)

Storage: 2-8C

Enzyme Commission (EC) Number: 3.5.1.52  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Application: Proteomics Grade PNGase F has been extensively purified and lyophilized from dilute potassium phosphate buffer to produce a stable product. The product is free from glycerol and other stabilizers, and contains very low levels of buffer salts. This highly purified material is excellent for N-linked deglycosylation of glycoproteins or glycopeptides in gel, in solution, or on blot membranes.

Application: Used to deglycosylate protein.

Biochem/physiol Actions: Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Unit Definition: One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.