Components: Biotinylated Phospho-Akt(pSer473)-specific Antibody Concentrate (Item C1); Phopho-Akt (pSer473) Lyophilized Positive Control Sample (Item K); Capture Antibody-Coated Microplate (Item A); 2X Cell Lysate Buffer (Item J); 5X Assay Diluent (Item E); HRP-conjugated Anti-Rabbit IgG Concentrate (Item D1); Phosphorylation ELISA Stop Solution (Item I); TMB One-Step Substrate Reagent (Item H); 20X Wash Buffer Concentrate (Item B)