Phosphatase, Alkaline shrimp, >=1,000 DEA units/mL, buffered aqueous glycerol solution, recombinant, expressed in proprietary host

Synonyms: Phosphatase, Alkaline from shrimp; Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

CAS Number: 9001-78-9

MDL No.: MFCD00131849

Storage: -20C

Enzyme Commission (EC) Number: 3.1.3.1  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

General description: Shrimp alkaline phosphatase is a heat labile, hydrolase enzyme. It is a high specific activity alkaline phosphatase purified from a recombinant source. There are two preferred sites for enzyme activity: 5' protruding, recessive and blunt 5'-termini. The enzyme is irreversibly heat inactivated at 65C for 15 minutes.

Application: Alkaline phosphatase was used to examine its role in the prevention of high-fat-diet-induced metabolic syndrome in mice. It also may be used in pasteurization process for milks used in dairy products.

Biochem/physiol Actions: Alkaline phosphatase is commonly responsible for dephosphorylation of nucleotides, proteins and alkaloids. The enzyme nonspecifically catalyzes the dephosphorylation of 5'-termini DNA and RNA phosphomonoesters reactions but it does not affect diphosphate or triphosphate linkages.

Physical form: solution in 50% glycerol containing 25 mM Tris-HCl, pH 7.5, 1 mM MgCl2

Unit Definition: One unit will hydrolyze 1 mumole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 C.

Analysis Note: Protein determined by biuret