Phosphatase, Alkaline from bovine intestinal mucosa, lyophilized powder, >=10 DEA units/mg solid

Synonyms: Alkaline phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

CAS Number: 9001-78-9

MDL No.: MFCD00131849

Storage: -20C

Enzyme Commission (EC) Number: 3.1.3.1  ( BRENDA | IUBMB )

EC Number: 232-631-4

UNSPSC Code: 12352204

Application: Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5'-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

Application: Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Product P7640 has been used to study the monoclonal alkaline phosphatase-anti-alkaline phosphatase (APAAP) complex.

Application: High specific activity grade recommended for antibody and protein conjugation.

Biochem/physiol Actions: The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Biochem/physiol Actions: Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols and it requires zinc, and magnesium or calcium divalent ions for activity.

Physical properties: Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Physical properties: Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Unit Definition: One DEA unit will hydrolyze 1 mumole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 C. (One glycine unit is equivalent to ~3 DEA units)

Analysis Note: Package sizes are based on DEA units