Phosphatase, Alkaline from bovine intestinal mucosa, BioUltra, buffered aqueous glycerol solution, >=6,500 DEA units/mg protein

Synonyms: Alkaline phosphatase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

CAS Number: 9001-78-9

MDL No.: MFCD00131849

Storage: 2-8C

Enzyme Commission (EC) Number: 3.1.3.1  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

General description: Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.

Application: Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5'-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

Application: High specific activity grade recommended for antibody and protein conjugation.

Application: Phosphatase alkaline from bovine intestinal mucosa has been used in a study to measure the hydrolysis kinetics of three polyfluorinated alkyl phosphate monoesters, differing in fluorinated chain length. It has also been used in a study to show that the developmentally controlled expression of the isosomes C-1 and C-2 indicate functional changes in intestinal alkaline phosphatase with tissue maturation.

Biochem/physiol Actions: The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Biochem/physiol Actions: Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A.

Physical form: Solution in 50% glycerol containing 5 mM Tris, 5 mM MgCl2 and 0.1 mM ZnCl2, pH 7.0

Physical properties: Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Physical properties: Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Unit Definition: One DEA unit will hydrolyze 1 mumole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 C. (One glycine unit is equivalent to ~3 DEA units)

Analysis Note: Package sizes are based on DEA units