Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.
Synonyms: Acyl-neuraminyl Hydrolase; Receptor-destroying enzyme; Sialidase
MDL Number: MFCD00131711
Storage: -20C
Application: Neuraminidase from Clostridium perfringens has been used in a study to assess binding with human T lymphocytes in sheep pretreated with neuraminidase. It has also been used in a study to investigate the effect of bile salts on the action of hydrolysis by neuraminidase.
Biochem Physiol Actions: The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 C.