SIGMA N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, =99.0% (AT)

Synonyms: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride; EDAC; EDC hydrochloride; WSC hydrochloride

Formula: C₈H₁₇N₃ · HCl

Formula Weight: 191.70

CAS No.: 25952-53-8

Purity: ≥99.0% (AT)

Storage Temperature: −20°C

Biochem/physiol Actions: Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Other Notes: Water soluble peptide coupling reagent;¹ Modification of carboxyl groups in proteins, review;² Synthesis of esters.³