Storage: -20C
UNSPSC Code: 12352200
Features and Benefits: Low contaminant DNA polymerasePrevents false positive PCR results from contaminating bacterial DNA
General description: MTP Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme has both 5'->3' DNA polymerase and exonuclease activities, is >=95 kDa by SDS-PAGE, and has no detectable endonuclease or 3'->5' exonuclease activities. Each lot of MTP Taq undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA.Contaminating DNA present in most other polymerase preparations often preclude or obscure the accurate interpretation of results, especially when targeting conserved sequences, e.g., bacterial 16S rRNA region. Through Sigma's proprietary DNA removal methods and strict quality control standards, we can ensure the absence of the most commonly found contaminant DNA. Each lot of MTP Taq is assayed using PCR and primers specific to (1) the conserved region of bacterial 16S rRNA, (2) the Taq expression vector, and (3) the human beta-actin gene.While MTP Taq ensures a high-quality, low contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10x MTP Taq Buffer with each tube of MTP Taq DNA Polymerase. Each lot of 10x MTP Taq Buffer undergoes the same strict quality control testing as MTP Taq DNA polymerase to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.
Application: MTP Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process that minimizes levels of contaminating DNA. The enzyme has 5'-3' DNA polymerase and exonuclease activities, is approximately 95 kD by SDS-PAGE, and has no detectable endonuclease or 3'-5' exonuclease activities. Contaminating DNA present in most other polymerase preparations often precludes or obscures the accurate interpretation of results, especially when targeting conserved sequences (e.g. bacterial 16S rRNA region). While MTP Taq is a high-quality, low-contaminant DNA polymerase for reliable PCR amplification, DNA contaminants can be introduced into PCR through a number of other reagents. To further minimize the risk of contaminant DNA during PCR, we include 10x MTP Taq buffer (Sigma product M 9943) with each tube of MTP Taq DNA Polymerase. Each lot of MTP Taq and 10x MTP Taq buffer undergoes the same strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA Polymerase.
Application: . Bacterial Genome Analysis. Pathogen Detection
Biochem/physiol Actions: MTP Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands.
Components: . MTP Taq DNA Polymerase (D7067). 10x MTP Taq Buffer (M9943)
Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA in 30 minutes at 74 C.
Other Notes: Store at -20 C. For convenience, 10x MTP Taq Buffer can be stored at room temperature.
Other Notes: Learn more about our offering of specialty enzymes at www.sigma-aldrich.com/specialtyenzymes.
Other Notes: View more detailed information on MTP Taq DNA Polymerase at www.sigma-aldrich.com/mtptaq.
RIDADR: NONH for all modes of transport
WGK Germany: 2