Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse, clone PC 10, ascites fluid

PCNA (proliferating cell nuclear antigen) is a member of the structurally and functionally conserved family of DNA sliding clamps (β clamps). They exist as ring-shaped complexes- homotrimers in eukaryotes, having pseudohexameric symmetry. A monomer of PCNA is composed of two similar globular domains, connected by an interdomain connecting loop, which is a long, and probably a flexible loop. These monomers organize themselves in a head-to-tail manner to form a ring, which has an inner positively charged surface composed of α helices and an outer surface of β sheets.

Synonyms: Monoclonal Anti-Proliferating Cell Nuclear Antigen; Anti-PCNA

Storage: -20C

Application: Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse has been used in:
•enzyme linked immuno sorbent assay (ELISA)
•immunoblotting
•immunohistology
•immunoprecipitation
•flow cytometry

Biochem Physiol Actions: Proliferating cell nuclear antigen (PCNA) is essential for DNA replication during S-phase. It is essential for cellular DNA synthesis. PCNA is required for leading strand synthesis in the SV40 system where it acts as an auxiliary protein for polymerase.