Anti-phospho-PAK1 (pThr212) antibody, Mouse monoclonal, clone PK-18, purified from hybridoma cell culture

MDL No.: MFCD03457569

Storage: -20C

UNSPSC Code: 12352203

General description: p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK. In the neurons, the reorganization of cytoskeleton during the neurite outgrowth is modulated by phosphorylation of PAK1 at Thr212 that regulates a specific signalling pathway Monoclonal Anti-Phospho-PAK1 (pThr212) reacts specifically with human PAK1 phosphorylated at Thr212 (68 kDa), and does not detect the unphosphorylated epitope.

Application: Anti-phospho-PAK1 (pThr212) may be used for immunoblotting at a working concentration of 0.2-0.4 mug/mL. The antibody is also suitable for indirect ELISA, immunocytochemistry and protein microarray.

Immunogen: synthetic peptide corresponding to amino acids 203-217 (pThr212) of human PAK1.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.