Anti-phospho-beta-Catenin (pSer33/pSer37) antibody, Mouse monoclonal, clone BC-22, purified from hybridoma cell culture

MDL No.: MFCD03457566

Storage: -20C

UNSPSC Code: 12352203

General description: CTNNB1 (catenin β 1) gene is mapped to human chromosome 3p21. The encoded protein β-catenin is localized to the nucleus.

Application: Monoclonal Anti-phospho-ß-Catenin (pSer33/pSer37) antibody is suitable for:. indirect ELISA. microarray. western blot at a dilution of 5-10μg/mL using whole cell extract of cultured human 293 cells treated with proteasome inhibitor MG132.

Biochem/physiol Actions: β-catenin is mapped on the short arm of chromosome 3. It is useful for differential diagnosis of Osteoblastoma (OB) and osteosarcoma (OS). β-catenin signaling pathway may contribute to cholangiocarcinoma (CCA) cell proliferation. It may serve as a prognostic marker for CCA progression and hence can be significant in the CCA therapy target.In Wnt/β-catenin signal pathway β-Catenin participates as a signal transduction molecule. The pathway is associated with development, tissue homeostasis and oncogenic transcription. Upregulation of the gene is associated with glioma cell proliferation. The protein encoded has dual function that regulates the coordination of cell-cell adhesion by linking cytoskeleton and cellular junctions and gene transcription. Wnt/β-catenin signal pathway is known to induce progression colon cancer progression.

Immunogen: synthetic phosphopeptide corresponding to the region that contains serines 33 and 37 of human beta-catenin.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity: The antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphos­phorylated peptide. It does not detect the unphos­phorylated or the Ser33 phosphorylated protein and does not recognize phosphorylated plakoglobin.