Monoclonal Anti-PARP antibody produced in mouse, clone C-2-10, ascites fluid

Synonyms: Monoclonal Anti-Poly[ADP-ribose] Polymerase

MDL No.: MFCD01322527

Storage: -20C

UNSPSC Code: 12352203

General description: PARP1 poly(ADP-ribose) polymerase 1) is also termed as ARTD1. It is an abundant nuclear protein that belongs to the PARP family. This gene is located on human chromosome 1q42.

General description: Monoclonal Anti-PARP (mouse IgG1) is produced by immunizing mice with purified calf thymus poly (ADPribose) polymerase as the antigen. This antibody recognizes a 116kDa protein which corresponds to PARP and the 85kDa apoptosis induced cleavage product of prICE (proteinase resembling interleukin 1b-converting enzyme) and CPP32 (cysteine protease). It also recognizes PARP from mouse, rat, hamster and primate sources, but fails to detect avian PARP.Poly (ADP-ribose) polymerase 1 (PARP1)/PARP is encoded by the gene mapped to human chromosome 1q42. It is a nuclear protein with molecular mass of 113kDa and is characterized with three functional domains. It belongs to the PARP superfamily.

Application: Monoclonal Anti-PARP (Cleaved-Asp214) antibody has been used in western blotting and immunohistochemical staining.

Biochem/physiol Actions: Poly (ADP-ribose) polymerase 1 (PARP1)/PARP participates in DNA repair. It facilitates homologous recombination. PARP controls transcription by regulating chromatin structure, altering DNA methylation patterns, acting as a co-regulator of transcription factors and interacting with chromatin insulators. It also plays an important role in the protection of cardiovascular system. PARP1 is also implicated in the maintenance of genomic stability.

Biochem/physiol Actions: PARP1 poly(ADP-ribose) polymerase 1) and its enzymatic product PAR controls cellular processes like DNA repair, chromatin remodeling, transcription and cell death. PARP1 regulates transcription. PARP1 can induce or block homologous recombination (HR).

Immunogen: calf thymus PARP. The epitope is found between amino acids 216-375 in the DNA-binding domain of PARP.

Physical form: Solution in mouse ascites fluid containing 0.02% sodium azide.

Specificity: The antibody reacts with PARP (116 kDa) and the 85 kDa apoptosis-induced cleavage product of prICE (proteinase resembling interleukin 1beta-converting enzyme) and caspase-3 (CPP32 cysteine protease).