Monoclonal Anti-p53 antibody produced in mouse, clone PAb1801, purified from hybridoma cell culture

Synonyms: Anti-p53 antibody, Mouse monoclonal; Anti-TRP53; Anti-Transformation-related protein 53

MDL No.: MFCD00164709

Storage: -20C

UNSPSC Code: 12352203

General description: Anti-p53 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma PAb1801 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. Anti-p53 antibody, Mouse monoclonal recognizes human p53. p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Application: Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Biochem/physiol Actions: Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Immunogen: recombinant human wild type p53. The epitope resides between amino acids 46-55 of human p53.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.