MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarly sized with apparent molecular weights of 280 kDa (MAP2a and b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears at levels equal to that of MAP2b. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and neurons have reached their mature morphology. MAP2 is known to promote microtubule assembly and to form side arms on microtubules. It also interacts with neurofilaments, actin and other elements of the cytoskeleton.
Synonyms: Monoclonal Anti-MAP2 antibody produced in mouse; MAP2 Antibody - Anti-MAP2 antibody, Mouse Monoclonal; Map2 Antibody
MDL Number: MFCD00164683
Storage: -20C
Application: May be used in various immunochemical techniques including immunoblotting, immunohistochemistry, and immunocytochemistry. Monoclonals antibodies to MAP2 are used to study the cytoskeleton structure in cells.By immunoblotting, a working antibody concentration of 1-2 mug/ml is recommended using a rat brain extract.
Biochem Physiol Actions: MAP2 (microtubule-associated protein 2) expression is monitored to predict the disease progression in glioma. MAP2 is critical in the formation of dendritic protrusions in astrocytes and oligodendrocytes. In eukaryotes, MAP2 is essential for spindle assembly and chromosome segregation.