Monoclonal Anti-HLA Class I Antigen-FITC antibody produced in mouse, clone W6/32, purified immunoglobulin, buffered aqueous solution

Synonyms: Monoclonal Anti-HLA Class I Antigen

Storage: 2-8C

UNSPSC Code: 12352203

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General description: The function of HLA class I molecules is to present antigen to CD8+ lymphocytes. They are involved in the regulation of positive and negative selection of CD8+ T cells by thymic epithelial cells during T cell maturation. These molecules are important in allograft rejection and in the linkage of certain haplotypes in autoimmune diseases. Expression of HLA class I antigens may be modulated in pathological states. Malignant cells may lose these antigens while hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may display enhanced reactivity. Increased expression of these antigens is described in muscle cells affected by muscular dystrophy, inflammatory myopathy and other neuro-muscular disorders.

Application: FITC Monoclonal Anti-Human HLA Class I Antigen may be used for immunochemical analysis of HLA Class I antigens; Studies of HLA-A,B,C expression on embryonic and neoplastic tissues; and/or the purification of HLA-A,B,C antigens.

Immunogen: human tonsil leukocyte membrane preparation.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Specificity: Recognizes the human HLA Class I antigen expressed on most human nucleated cells. The antibody reacts with a monomorphic epitope on the 44-45 kDa alpha-chain polypeptide of human class I HLA molecules (HLA-A,B,C). The antibody may be used in immunoprecipitation, complement mediated cytotoxicity, flow cytometry and immunohistochemical staining of acetone-fixed, frozen sections and cell smears. It cross-reacts with cells from African and Asian apes and old world monkeys. No reactivity is seen with cells from most new world monkeys or from non-primates. The epitope recognized by this antibody is sensitive to routine formalin fixation and paraffin embedding.4th Workshop: code no. P15th Workshop: code nos. BP166, BP288, BP407, B 005

Preparation Note: Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.