MDL No.: MFCD02096517
Storage: -20C
UNSPSC Code: 12352203
General description: The antibody reacts with various forms of the H+/K+ ATPase beta subunit. It binds an epitope within amino acids 1-13 or 15-28 located on the cytoplasmic side of the beta subunit. The antibody has been shown to inhibit the enzymatic activity of the H+/K+-ATPase and to alter the affinity of the cytoplasmic K+ binding site. It may be used to localize and detect H+/K+ ATPase beta subunit.
General description: The H+/K+-ATPase is a gastric proton pump that is a member of the P-type cation-transporting adenosine 5'-triphosphatase (ATPase) family. H+/K+-ATPase consists of a large transmembrane, catalytic alpha subunit, that contains sites for ATP binding and phosphorylation. This ATPase also has a smaller glycoprotein (beta) subunit which may play a role in maintaining the structural and functional integrity of the complex. Mouse monoclonal anti-H+/K+ ATPase (beta subunit) binds to H+/K+-ATPase beta subunit in cow, dog, pig, rabbit, mouse, ferret and rat tissues. By immunoblotting, the antibody detects various forms of the beta subunit, including a 60-80kDa glycosylated protein, a 52kDa beta subunit precursor and the 34kDa core peptide.
Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)
Application: Mouse monoclonal anti-H+/K+ ATPase (beta subunit) antibody can be used for indirect immunofluorescence (1:2,000), chemiluminescent western blot (1:4,000), and immunohistochemical applications.
Immunogen: core 34 kDa H+/K+ ATPase beta subunit from deglycosylated pig gastric microsomes.
Physical form: Solution in phosphate buffered saline containing 0.05% sodium azide.
RIDADR: NONH for all modes of transport