Synonyms: Anti-ddddk; Anti-dykddddk
MDL No.: MFCD00282246
Storage: -20C
UNSPSC Code: 41106000
Features and Benefits: Antibody BioguaranteeEvaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
General description: Monoclonal ANTI-FLAG(R) M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.
Application: Suitable for western blotting.Browse additional application references in our FLAG(R) Literature portal.
Application: Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca2+-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.
Immunogen: FLAG; peptide sequence DYKDDDDK
Physical form: Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)
Preparation Note: Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.
Other Notes: Antibody is not calcium dependent.
RIDADR: NONH for all modes of transport