Synonyms: Anti-ddddk; Anti-dykddddk
MDL No.: MFCD00212941
Storage: -20C
UNSPSC Code: 41106000
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General description: The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.
Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Chromatin immunoprecipitation (1 paper)Immunofluorescence (1 paper)
Biochem/physiol Actions: Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.
Immunogen: FLAG; peptide sequence DYKDDDDK
Physical form: Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).
Preparation Note: Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.
Other Notes: M1 antibody binding is calcium dependent.
RIDADR: NONH for all modes of transport
WGK Germany: 3
R Codes: 21/22
S Codes: 36/37
IOD Codes: Xn