Monoclonal Anti-Fibronectin antibody produced in mouse, clone IST-3, ascites fluid

MDL No.: MFCD00162291

Storage: -20C

UNSPSC Code: 12352203

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General description: Mouse monoclonal clone IST-3 anti-Human Fibronectin antibody recognizes an epitope located within the fourth type-three repeat of human plasma fibronectin. The antibody localizes plasma and cellular fibronectin both in its natural and denatured-reduced forms (240 kDa) using immunoblotting, RIA, ELISA, immunocytochemistry and immunohistochemistry. This product cross-reacts with goat, sheep, bovine, dog, rabbit and chicken fibronectin.

General description: Fibronectin (FN) is an extracellular matrix protein composed of two nearly-identical disulfide-bound polypeptides with typical molecular weights of 220-280 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and antibodies to either form usually cross-react. Careful analysis of the FN molecule indicates that it contains several functionally and structurally distinct domains which may bind to cell surfaces and to a variety of molecules such as collagen, heparin, gelatin, fibrin and DNA. FN's play an important role in diverse biological phenomena including cell adhesion, cell migration, hemostasis and thrombosis, wound healing and the ability to induce a more normal phenotype in transformed cells.

Application: Mouse monoclonal clone IST-3 anti-Human Fibronectin antibody may be used for the localization of fibronectin using various immunochemical assays including ELISA, immunoblot, dot blot, RIA, immunocytochemistry and immunohistochemistry. It may be used for the specific labeling of fibronectin in cultured cells and in frozen tissue sections. Staining of formalin-fixed, paraffin-embedded sections may require prolonged proteolytic unmasking (e.g., by trypsin). Monoclonal antibodies reacting specifically with FN may be used to localize FN in human cell cultures, in tissue sections and in plasma. They may also be used for immunoaffinity purification and immunoprecipitation of cellular fibronectin. In addition, these antibodies may be used to specifically modulate the functional activity of FN.

Immunogen: human plasma fibronectin