Monoclonal Anti-Fas (CD95/Apo-1) antibody produced in mouse, clone DX2, purified immunoglobulin, buffered aqueous solution

Synonyms: Monoclonal Anti-Fas; Anti-Apo-1; Anti-CD95

Storage: -20C

UNSPSC Code: 12352203

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General description: Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well studied receptors are CD95/Fas/Apo-1 (apoptosis inducing protein 1) and tumor necrosis factor receptor 1 (TNFR1). Apoptosis mediated by either of these results in activation of the caspases. However, Fas-mediated death occurs much more rapidly than that triggered by TNFR1. Human Fas/CD95/Apo-1 is a single transmembrane glycoprotein receptor (325 amino acids, 45-48 kDa).

Application: Monoclonal Anti-Fas (CD95/Apo-1) antibody is suitable for apoptosis treatment of Jurkat cells to study the contribution of channel type into the net K+ flux. It is also suitable for flow cytometry at a concentration of 4-20μg/mL using cultured human Burkitt's lymphoma Raji cells.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Biochem/physiol Actions: Human CD95/Fas/Apo-1 antigen is a single transmembrane glycoprotein receptor of 325 amino acids (45-48 kDa) which activate cell apoptosis. The action of Fas is mediated via FADD (Fas-associated death domain)/ MORT1, an adapter protein that has a death domain at its C-terminus and binds to the cytoplasmic death domain of Fas. APO-1/Fas(CD95) comprises of a death domain (DD) within the cytoplasmic region which triggers apoptosis upon binding of their cognate ligands. Once it is activated, APO-1/Fas(CD95) further aggregates its intracellular death domains which leads to the recruitment of two key signaling proteins followed by the formation of death-inducing signaling complex. These complex crosslinks through its C-terminal DD with APO-1/Fas receptors and engage caspase-8 via its N-terminal death effector domain (DED) to the DISC.

Immunogen: murine L cells transfected with a human Fas/CD95 cDNA.

Physical form: Solution from a bioreactor culture supernatant in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity: Reacts specifically with the functional epitope of human Fas (CD95/Apo-1) antigen. By immunoblotting, the clone recognizes denatured, non-reduced recombinant human Fas (amino acid residues 1-173). The antibody is reactive in flow cytometry, and may be reactive in the induction of apoptosis.