Monoclonal Anti-DNP-Alkaline Phosphatase Conjugate antibody produced in mouse, clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution

Synonyms: Anti-Dinitrophenyl; Monoclonal Anti-DNP

Storage: 2-8C

UNSPSC Code: 12352203

General description: Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.

Application: The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

Application: Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.

Application: The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.

Immunogen: DNP-KLH conjugate

Physical form: Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 50% glycerol and 15 mM Sodium azide

Analysis Note: Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.