Monoclonal Anti-Dinitrophenyl antibody produced in mouse, IgE isotype, ~1 mg/mL, clone SPE-7, affinity purified immunoglobulin, buffered aqueous solution

Synonyms: Anti-Dinitrophenyl; Anti Dnp Ige; Anti-DNP IGE - Monoclonal Anti-Dinitrophenyl antibody produced in mouse; Anti-Dnp Ige; Monoclonal Anti-DNP

Storage: -20C

UNSPSC Code: 12352203

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General description: Dinitrophenyl (DNP) is a hapten that is often used for labeling primary or secondary probes in immunological assays. Hence, anti-DNP antibody is a useful tool for the detection and analysis of target molecules. Monoclonal Anti-DNP antibody is specific for DNP.

Application: The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Application: Monoclonal Anti-Dinitrophenyl antibody is suitable for use in flow cytometry and immunocytochemistry.

Application: The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.

Immunogen: DNP-KLH conjugate

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.