Monoclonal Anti-Collagen, Type IV antibody produced in mouse, clone COL-94, ascites fluid

Synonyms: Collagen Iv Antibody; Collagen Iv Antibody - Monoclonal Anti-Collagen, Type IV antibody produced in mouse

MDL No.: MFCD00162240

Storage: -20C

UNSPSC Code: 12352203

General description: The composition of the extracellular matrix of all vertebrates is dominated by a class of molecules known as collagens, each with a unique feature suited to its function and location. Type IV collagen is a major constituent of basement membranes. This molecule is a continuous triple helix, about 400 nm in length, composed of at least two different αchain subunits termed α1 (IV) and α2 (IV) which may be composed of the same or different molecules. Upon proteolysis, fragments of 125 kDa, 95 kDa, and 60 kDa are released from a parent α1 (IV) chain, whereas fragments of 115 kDa, 80 kDa, and 45 kDa apparently originate from a distinct α2 (IV) chain. Collagens are known to have multiple functions. They are involved in tissue differentiation, morphogenesis and repair; play a role in the maintenance of tissue architecture; and form the filtration barrier of blood vessels.

Application: Collagen, Type IV monoclonal antibody may be used in immunoassays such as immunocytochemistry, dot blot and ELISA. The antibody has been used for immunogold staining for electron microscopy, to visualize type IV collagen, in patients with coeliac disease. A minimum working dilution of 1:500 is determined by immunoperoxidase staining of formalin-fixed, paraffinembedded and enzyme-treated human tissue.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunohistochemistry (1 paper)Western Blotting (1 paper)

Immunogen: human collagen type IV

Specificity: Using immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the alpha1 and/or alpha2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C.