Monoclonal Anti-CD4 antibody produced in mouse, clone Q4120, purified from hybridoma cell culture

Synonyms: Anti-CD4 antibody, Mouse monoclonal

Storage: 2-8C

UNSPSC Code: 12352203

Application: Monoclonal Anti-CD4 antibody produced in mouse is suitable for flow cytometry at a concentration of 5muL using 1 x 106 cells.

Biochem/physiol Actions: CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T-helper cells, monocytes, macrophages and dendritic cells. CD4 gene is located at chromosome 12p13. Stable CD4 cell expression and local immune activation may promote viral replication, genital shedding and risk of sexual HIV transmission in the lower female genital tract. Patients with infectious and chronic inflammatory diseases are detected with higher level of soluble CD4 (sCD4). It may act as an important parameter for rheumatoid arthritis (RA) disease progression. CD4+ T cells are critical for the pathogenesis of myocarditis. CD4+ CD25+ regulatory T cells (Tregs) may control the development and progression of autoimmunity by suppressing auto-reactive effector T cells.

Immunogen: CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity: Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11

Target description: CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56lck protein tyrosine kinase.