Monoclonal Anti-CD31 (PECAM-1) antibody produced in mouse, clone WM-59, purified immunoglobulin, buffered aqueous solution

Synonyms: Monoclonal Anti-CD31; Anti-PECAM-1

Storage: -20C

UNSPSC Code: 12352203

General description: Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-beta1. Its reactivity with platelets is enhanced following their washing. 5th Workshop: code No. P025

General description: The human PECAM-1 antigen (Platelet Endothelial Cell Adhesion Molecule, CD31, endoCAM. gpIIa, hec7) is a 130-140 kD single chain integral membrane glycoprotein member of the immunoglobulin gene superfamily of cell adhesion molecules. Human PECAM-1 (CD31) is expressed on platelets, myeloid cells, B lymphocytes, certain T lymphocyte cell subsets, bone marrow precursor cells and NK cells. PECAM is abundantly expressed in endothelial cells. It becomes localized to the intracellular junctions in monolayers of cultured endothelial cells. PECAM-1 (CD31) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities. It plays a major role in the transmigration of monocytes, neutrophils and NK cells between the endothelial cell junctions into the subendothelial matrix. PECAM-1 (CD31) is possibly involved in some of the interactive events taking place during cardiovascular development inflammation, thrombosis, wound healing and angiogenesis.

Application: Mouse monoclonal clone WM-59 anti-human PECAM-1 (CD31) may be used for studies of PECAM-1 function in cell-cell interactions, for the detection and enumeration of CD31 cells in blood and tissues, and for the isolation of PECAM-1 by immunoaffinity chromatography. Monoclonal Anti-Human PECAM-1 (CD31) was shown to increase the rate of homotypic aggregation induced in U937 cells by TGFbeta1. Its binding to platelets was reported to be enhanced following their washing and concomitant activation.

Immunogen: human cell line RC-2A, originally derived from myeloid leukemia cells.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4

Specificity: Recognizes the CD31 (PECAM-1, endoCAM, gpIIa, hec7), human cell surface integral membrane glycoprotein. The antibody increases the rate of homotypic aggregation induced in U937 cells by TGF-beta1. Its reactivity with platelets is enhanced following their washing.5th Workshop: code No. P025

Target description: Human CD31 (PECAM-1) is expressed on platelets, endothelial cells, myeloid cells, B lymphocytes, and certain T lymphocyte subsets. CD31 (PECAM-1) functions in homophilic and heterophilic cell-cell adhesion and cell signaling activities.

Preparation Note: Product filtered through a 0.2 mum filter