Monoclonal Anti-CD16-FITC antibody produced in mouse, clone 3G8, purified immunoglobulin, buffered aqueous solution

MDL No.: MFCD00675220

Storage: 2-8C

UNSPSC Code: 12352203

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General description: Human CD16 or FCγRIII is a 50-70kDa membrane glycoprotein expressed by neutrophils, eosinophils, cultured monocytes, natural killer (NK) cells, tissue macrophages subpopulation, and on a small subset of T cells that binds aggregated but not monomeric human IgG. It is also known as FcR3, the low affinity receptor for complexed IgG. It comprises of two truncated Ig-like cytoplasmic domains.

Application: Monoclonal Anti-CD16-FITC antibody is suitable for receptor blocking in flow cytometry analysis.

Biochem/physiol Actions: Human CD16 plays a pivotal role in immune responses by linking the humoral immune system with cellular effector functions. CD16 plays an important role in NK-cell function and in peripheral blood mononuclear cells (PBMCs). In the FcyRIII-expressing lymphocytes the NK cell activity is highly influenced through FcyRIII. CD16 exists as a transmembrane form in NK cells, macrophages and cultured monocytes with a 25 amino acid cytoplasmic tail which further connects with other receptors. The transmembrane form binds complexed IgG and mediates phagocytosis and antibody-dependent cellular cytotoxicity (ADCC).

Immunogen: viable human polymorphonuclear cells.

Physical form: Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity: Recognizes the human CD16 antigen expressed on natural killer (NK) cells, granulocytes and a macrophage subpopulation. The antibody reacts with both NA1 and NA2 neutrophils and NK lymphocytes. It strongly labels neutrophils in tissue frozen sections. Liver Kupffer cells are weakly stained. The epitope recognized by the antibody seems to reside near the IgG binding site of FCgamma-RIII. The antibody functionally blocks binding of soluble immune complexes to granulocytes and inhibits E-IgG rosettes. It induces Ca2+ increase in neutrophils.

Target description: CD16 antigen (also known as the low affinity receptor for complexed IgG, or Fcγ-RIII) expressed on natural killer (NK) cells, a macrophage sub-population and weakly on granulocytes. The human CD16 molecule exhibits two truncated Ig-like domains. In NK cells and macrophages, a trans-membrane form (50-80 kDa) with a 25 amino acid cytoplasmic tail is present. This form is non-covalently associated with other receptors (FcεRIγ or the TcRξ chain). The human CD16 molecule in granulocytes is a glycosyl-phosphatidyl-inositol (GPI)-linked form.

Preparation Note: Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.