Monoclonal Anti-Cathepsin L antibody produced in mouse, clone 33/2, ascites fluid

Synonyms: Anti-CATL; Anti-CTSL; Anti-MEP

MDL No.: MFCD01321805

Storage: -20C

UNSPSC Code: 12352203

General description: Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. They are normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Western Blotting (1 paper)

Application: Monoclonal Anti-Cathepsin L is suitable for ELISA, immunoblotting, immunohistochemistry (frozen sections) and for the suppression of the malignant growth of myeloma cells.

Biochem/physiol Actions: Cathepsin L (EC 3.4.22.15) is responsible for most of the intralysosomal protein breakdown of normal cells. Monoclonal Anti-Cathepsin L (mouse IgG1 isotype) is derived from the 33/2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with procathepsin L isolated from the human lung cancer line EPLC 32 M1. Monoclonal Anti-Cathepsin L reacts specifically with the native and denatured forms of human cathepsin L (25 kDa) and procathepsin L (42 kDa).

Immunogen: procathepsin L isolated from the human lung cancer cell line EPLC 32M1.

Specificity: Reacts specifically with native and denatured forms of human cathepsin L and procathepsin L. Recognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule). The antibody may be used for the suppression of the malignant growth of myeloma cells. Cross-reactivity is observed with rat cathepsin L (strong), mouse procathepsin L (weak) and mink cathepsin and procathepsin L. Does not cross-react with human cathepsin types B, D, H and S, procathepsins B and D, rat cathepsin B, and mouse procathepsins B and D.