Monoclonal Anti-Albumin antibody produced in mouse, clone HSA-9, ascites fluid

MDL No.: MFCD00162390

Storage: -20C

UNSPSC Code: 12352203

General description: Albumin is a transport protein that binds a broad range of ligands such as fatty acids, bilirubin, hemin, drugs, amino acids and ions . Albumin may also function as a zinc carrier protein and consequently regulate physiological processes . Monoclonal Anti-Albumin antibody is specific for human serum albumin and shows cross reactivity with rhesus monkey and baboon albumins when tested in an immunoblot procedure under non-reducing conditions. The antibody does not react with bovine, cat, catfish, chicken, dog, donkey, gibbon, goat, guinea pig, hamster, horse, marmoset, mouse, pig, pigeon, rabbit, rat, sheep or turkey serum albumin.

General description: Albumin, the major protein produced by liver cells, represents more than half of the total protein content of human serum. Many other body fluids also contain albumin. Three major functions for serum albumin have been proposed: maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease.

Application: Western blot analysis of monkey retinal protein extracts were performed using the mouse monoclonal anti-albumin antibody at 1:20000 and overnight incubation at 4 degrees. Immunohistochemistry was performed on frozen monkey retina sections with the mouse monoclonal anti-albumin antibody at 1:1000. Uptake of albumin by LLC-PK1 cells was monitored by immunofluorescence using the mouse monoclonal anti-albumin antibody. Cells were first fixed in 4% formaldehyde the incubated with the antibody for 1 hour at room temperature.

Application: Monoclonal Anti-Albumin antibody is suitable for use in indirect ELISA (1:500)

Immunogen: Human platelets

Physical form: The product is provided as ascites fluid with 0.1% sodium azide as a preservative.