The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis.
Synonyms: Monoclonal Anti-Actin
Storage: -20C
Application: Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.
Biochem Physiol Actions: Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal region of actin appears to be a major antigenic region, and may be involved in the interaction of actin with other proteins such as myosin. Among the six isoforms of actin, four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. There are three a-actins (skeletal, cardiac, and smooth muscle), one ß-actin (ß-nonmuscle), and two ?-actins (?-smooth muscle and ?-nonmuscle). Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their NH2-terminal residues.The actin in cells of various species and tissues are very similar in their immunological and physical properties characterized by electrophoresis and amino acid sequence analysis.(from bulletin) They mediate cytoskeletal remodeling during T cell activation. Mutation in the ?-actin and ß-actin gene is associated with a developmental disorder called Baraitser-Winter syndrome. Mutation in the ?-actin leads to hearing loss.