a-Actinin is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, a-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. The gene encoding a-actinin is mapped to the human chromosome 11q13.2. a-Actinin belongs to the spectrin protein superfamily.a-Actinin dimerizes in an antiparallel fashion via interaction of the central rod domains. Four isoforms are found in human and mice. a-actinin-1 is ubiquitously expressed and located primarily in focal adhesions, whereas a-actinin-4 is present in certain membrane ruffles and seems to play a role in endocytosis and tumor cell motility.ACTN1 (a-actinin-1) and ACTN4 (a-actinin-4) encodes non-muscle proteins, while ACTN2 (a-actinin-2) and ACTN3 (a-actinin-3) encodes muscle proteins.Monoclonal Anti-a-Actinin (Sarcomeric) shows wide reactivity, as it binds to human, bovine, pig, sheep, rabbit, goat, hamster, cat, rat, mouse, dog, chicken, lizard, snake, frog and fish a-actinin.
Synonyms: Anti-1110008F24Rik; Anti-ACTN2; Anti-Actinin alpha2; Anti-CMD1AA; Anti-MGC107582; Sarcomeric Alpha Actinin Antibody; Sarcomeric Alpha Actinin Antibody - Anti-alpha-Actinin (Sarcomeric) antibody, Mouse monoclonal
Storage: -20C
Application: Western blot analysis of transfected HEK-293 cells were performed to evaluate actinin expression using monoclonal mouse anti-actinin antibody.
Biochem Physiol Actions: a-Actinin maintains the structure of cytoskeletal organization and regulates muscle contraction. a-Actinin functions as an actin crosslinker and promotes cell migration. Mutations in the a-actinin gene is observed in heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.