Synonyms: Mucopeptide N-acetylmuramoylhydrolase; Muramidase
CAS Number: 12650-88-3
MDL No.: MFCD00131557
Storage: -20C
Enzyme Commission (EC) Number: 3.2.1.17 ( BRENDA | IUBMB )
UNSPSC Code: 12352204
General description: Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.
Application: Suitable for the purification of both DNA and protein from bacteria.
Application: Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions: Lysozyme hydrolyzes beta(1->4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Physical form: Solution in 50% glycerol, 25 mM Sodium Acetate
Suitability: Tested for suitability with CelLytic(TM) B in protein extraction from bacteria.
RIDADR: NONH for all modes of transport
WGK Germany: 2