Synonyms: LuminoCt(R) qPCR ReadyMix(TM); LuminoCt®
Storage: -20C
UNSPSC Code: 41106300
Features and Benefits: . Assay results in as little as 25 minutes. Unsurpassed accuracy, precision, and sensitivity. Virtually eliminates the need to optimize assay parameters
Application: LuminoCt qPCR ReadyMix is designed to deliver unsurpassed assay speeds without sacrificing accuracy, precision, or sensitivity. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers and probes. Inclusion of JumpStart(TM) Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.. Kit is designed to perform probe-based qPCR assays on commonly available real-time instrument platforms.. ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays.. Kit is not compatible with qPCR instruments that utilize glass capillary tubes.
Other Notes: . Commercially available 5' nuclease assays (e.g., TaqMan(R) Assays) are compatible with LuminoCt ReadyMix.. At a minimum, use software to design primers and probes for assays and ensure that qPCR amplicons are<200 bp.. sigma recommends designing primers and probes with beacon designer softwareto order primers and probes from sigma, visit www.sigma.com ligos<>
Components: 100X ROX internal reference dye
RIDADR: NONH for all modes of transport
WGK Germany: 1