Leptomycin B solution from Streptomyces sp., >=95% (HPLC), Supplied in methanol: water (7:3)

CAS Number: 87081-35-4

Formula: C33H48O6

Formula Weight: 540.73

Linear Formula: C33H48O6

MDL No.: MFCD06795848

Purity: >=95% (HPLC)

Storage: -20C

UNSPSC Code: 12352200

General description: Leptomycin B is an anti-fungal antibiotic, anti-tumor cytotoxin that inhibits CRM1-dependent, NES-dependent nucleo-cytoplasmic translocation (nuclear export inhibitor). NES containing proteins include HIV-1 REV; actin, c-Abl, cyclin B1, MDM2/p53, I?B, MPF, PKA and MEK.

Biochem/physiol Actions: Leptomycin B is an unsaturated, branched-chain fatty acid, and is an important tool in the study of nuclear export. Leptomycin B is a specific inhibitor of proteins containing nuclear export signal. Leptomycin B inhibits nucleo-cytoplasmic translocation of molecules such as the HIV-1 Rev protein and Rev-dependent export of mRNA. The addition of very small amounts to fibroblasts causes accumulation of MEK in the nucleus. Other proteins that are influenced by leptomycin B are actin, c-Abl, cyclin B1, MDM2/p53, IkappaB, MPF, and PKA. The suggested inhibition mechanism involves the direct binding of leptomycin B to CRM1, which blocks the binding of CRM1 to proteins containing the nuclear export signal, via the interaction with cysteine residue in CRM1 control conserved region.

Other Notes: The volume in each sku is 0.1 mL with 10-20% overpack.