Storage: -20C
UNSPSC Code: 12352200
General description: Sigma's Lambda DNA Hind III Digest contains 8 fragments from 125 - 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application: Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Components: Sigma's Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Preparation Note: Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.
Other Notes: Contains 8 fragments, 125-23,130 bp
Other Notes: For optimal resolution, the recommended agarose gel concentration is 0.75%.
RIDADR: NONH for all modes of transport