Synonyms: Lactate; (S)-Lactate: NAD+ oxidoreductase; L-LDH; LAD; LD
CAS Number: 9001-60-9
MDL No.: MFCD00131466
Storage: 2-8C
Enzyme Commission (EC) Number: 1.1.1.27 ( BRENDA | IUBMB )
EC Number: 232-617-8
UNSPSC Code: 12352204
General description: Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.Isoelectric point: 8.4-8.6Optimal pH : 7.5 .
Biochem/physiol Actions: Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.
Physical form: Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0
Unit Definition: One unit will reduce 1.0 mumole of pyruvate to L-lactate per min at pH 7.5 at 37 C.
Analysis Note: Protein determined by biuret.
RIDADR: NONH for all modes of transport
WGK Germany: 3