JumpStart(TM) REDTaq(R) ReadyMix(TM) Reaction Mix, for High-throughput PCR of complex templates

MDL No.: MFCD00166026

Storage: -20C

UNSPSC Code: 41106300

Features and Benefits: . JumpStart Taq DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity . JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration . REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications . Inert red dye allows for easy verification that reagent has been mixed properly. Other loading dyes not necessary. Aliquots may be directly loaded onto an agarose gel after the reaction

General description: JumpStart REDTaq ReadyMix PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma's ReadyMix and REDTaq. The mix includes Sigma's JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2x optimized reaction concentrate. Add 25 muL of the ReadyMix to primers, template, and water to a final reaction volume of 50 muL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored.The red dye allows quick visual confirmation the enzyme has been added and properly mixed. After PCR, an aliquot may be loaded directly onto an agarose gel without the addition of loading buffers. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired.Using JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.

General description: JumpStart REDTaq ReadyMix PCR Reaction Mix for High Throughput PCR is formulated with REDTaq Genomic DNA polymerase for amplification of more complex templates and genomic templates. This mix contains optimized enzyme and dye concentrations to provide increased length and yield on the more difficult templates.

Application: . For multiplex PCR. For amplification with reduced non-specific amplification. For high throughput PCR amplification of complex templates

Unit Definition: One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 C.

Other Notes: View more detailed information on JumpStart REDTaq and ReadyMix products at www.sigma-aldrich.com/hotstart.