Purity: ≥95% (SDS-PAGE)
Analysis Note: The specific activity was determined by the dose-dependent simulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Preparation Note: HumanKine IL-4, expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 14-19 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Legal Information: HumanKine is a registered trademark of HumanZyme Inc.
Storage: −20°C
Biochem/physiol Actions: Interleukin-4 is a complex glycoprotein released by a subset of activated T cells, mast cells, and basophils. Human and mouse IL-4 share a 50% amino acid sequence homology, but their biological actions are species specific. IL-4 is a pleiotropic cytokine that has profound effects on the growth and differentiation of immunologically competent cells. IL-4 regulates differentiation of na+»ve CD4+ T cells to the TH2 type. IL-4 also regulates IgE and IgG1 production by B cells.
Empirical Formula (Hill Notation): C8H16O2