Components: Human Osteopontin Antibody-coated ELISA Plate (Item A); Lyophilized Human Osteopontin Protein Standard (Item C); Biotinylated Human Osteopontin Detection Antibody (Item F); ELISA 5X Assay/Sample Diluent Buffer E (Item E2); HRP-Streptavidin (Item G); ELISA Stop Solution (Item I); ELISA Colorimetric TMB Reagent (HRP Substrate, Item H); 20X Wash Buffer (Item B)