Synonym(s): HGF; Hepatopoietin A; Scatter Factor
Purity: ≥95% (SDS-PAGE)
Physical form: Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine.
Analysis Note: The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.
Other Notes: The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.
Storage: −20°C
Biochem/physiol Actions: Hepatocyte growth factor is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.
Empirical Formula (Hill Notation): C6H15N