SIGMA Guanidine hydrochloride PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production.

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade.

Synonyms: Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride

Molecular Formula: CH5N3 · HCl

Molecular Weight: 95.53

Linear Structural Formula: NH2C(=NH)NH2 · HCl

MDL Number: MFCD00013026

Melting Point: 180-185 C (lit.)

Density: 1.3 g/cm3 (lit.)

Application: Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.