Synonyms: Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride
Formula: NH2C(=NH)NH2 · HCl
Formula Weight: 95.53
CAS No.: 50-01-1
Purity: ≥99.0% (AT)
Application: Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Anion Traces: sulfate (SO42-): ≤50 mg/kg
Assay: ≥99.0% (AT)
Cation Traces: K: ≤50 mg/kg
Ign. Residue: ≤0.1% (as SO4)
MP: 180-185°C(lit.)
Solubility: H2O: soluble6 M at 20°C, clear, colorless