Synonyms: Aminoformamidine hydrochloride; Aminomethanamidine hydrochloride; Guanidinium chloride
Molecular Formula: CH5N3 · HCl
Molecular Weight: 95.53
Linear Structural Formula: NH2C(=NH)NH2 · HCl
MDL Number: MFCD00013026
Purity: >=99% (titration)
Melting Point: 180-185 C (lit.)
Density: 1.3 g/cm3 (lit.)
Application: Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Biochem Physiol Actions: Guanidine hydrochloride (GdnHCl) is hydroscopic and a small molecule. It is a potent denaturant of enzymes, which results in conformational changes in the enzyme, leading to complete loss of enzyme activity. GdnHCl is involved in blocking the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It is involved in denaturing alkaline phosphatase (ALPase) in Haliotis diversicolor. GdnHCl leads to the deactivation of enzymes like papain and aminoacylase. GdnHCl, at higher concentrations, is effective in virus inactivation, such as herpes simplex virus 1 (HSV-1).
Legal Information: Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
Abs.: A6M/260 nm, H2O ≤0.10
Assay: ≥99% (titration)
MP: 180-185°C(lit.)
Quality Level: ELITE
Solubility: H2O: soluble6 M