Glucose Oxidase from Aspergillus niger, Type VII, lyophilized powder, >=100,000 units/g solid (without added oxygen)

Synonyms: beta-D-Glucose:oxygen 1-oxidoreductase; G.Od.; GOx

CAS Number: 9001-37-0

MDL No.: MFCD00131182

Storage: -20C

Enzyme Commission (EC) Number: 1.1.3.4  ( BRENDA | IUBMB )

EC Number: 232-601-0

UNSPSC Code: 12352204

General description: Molecular Weight: 160 kDa (gel filtration)pI: 4.2Extinction coefficient: E1% = 16.7 (280 nm)Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation.Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates. The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for beta-D-glucose with a KM of 33-110 mM.Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide.Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes beta-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for beta-D-glucose, solutions of D-glucose can be quantified as alpha-D-glucose will mutorotate to beta-D-glucose as the beta-D-glucose is consumed by the enzymatic reaction.

Application: Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors.

Biochem/physiol Actions: Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.

Physical form: Lyophilized powder containing phosphate buffer salts and sodium chloride

Quality: May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.

Unit Definition: One unit will oxidize 1.0 mumole of beta-D-glucose to D-gluconolactone and H2O2 per min at pH 5.1 at 35 C, equivalent to an O2 uptake of 22.4 mul per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.

Analysis Note: Protein determined by biuret.