Components: 96-well plate coated with secondary antibody (Item A); EIA Glucagon Peptide standard (Item C); Anti-Glucagon Detection Antibody (Item N); Biotinylated Glucagon Peptide (Item F); Glucagon Positive Control Sample, Lyophilized (Item M); EIA 5X Assay Diluent B (Item E); EIA Assay Diluent A (Item D); HRP-Streptavidin; Stop Solution (Item I); TMB Substrate solution (Item H); 20X Wash Buffer (Item B)