Fructose-6-phosphate Kinase from Bacillus stearothermophilus, Type VII, lyophilized powder, >=50 units/mg protein

Synonyms: 6-Phosphofructokinase; ATP:D-fructose 6-phosphate 1-phosphotransferase

CAS Number: 9001-80-3

MDL No.: MFCD00131107

Storage: -20C

Enzyme Commission (EC) Number: 2.7.1.11  ( BRENDA | IUBMB )

EC Number: 232-633-5

UNSPSC Code: 12352204

General description: Bacillus stearothermophilus phosphofructokinase (BsPFK) is a homotetramer that is allosterically inhibited by phosphoenolpyruvate (PEP), which binds along one dimer-dimer interface.

Application: Fructose-6-phosphate Kinase from Bacillus stearothermophilus was shown to interact with neuronal nitric oxide synthase (nNOS) causing a defect in glycolytic metabolism and increased fatigability in dystrophic muscle.

Biochem/physiol Actions: Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.

Physical form: Lyophilized powder containing phosphate buffer salt

Unit Definition: One unit will convert 1.0 mumole of fructose 6-phosphate and ATP to fructose 1,6-diphosphate and ADP per minute at pH 9.0 at 30 C.